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Protein Quantification (Bradford Assay)
2:40
YouTubeAbnova
Protein Quantification (Bradford Assay)
( http://www.abnova.com ) - Bradford assay is a rapid and accurate method to determine the concentration of protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions ...
146.8K viewsApr 7, 2010
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